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Partec cyflow ml flow cytometry technique
Cyflow Ml Flow Cytometry Technique, supplied by Partec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry+technique/cytometry+flow/pm39090674-159-11-16
Average 86 stars, based on 1 article reviews
cyflow ml flow cytometry technique - by Bioz Stars, 2026-10
86/100 stars

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Flow Cytometry:

Article Title: miR-181b and miR-204 suppress the VSMC proliferation and migration by downregulation of HCK.
Article Snippet: Background: VSMC proliferation and migration pathways play important roles in plaque formation in the vessel stenosis and re-stenosis processes.. The microRNAs affect the expression of many genes that regulate these cellular processes.. The aim of this study was to investigate the effects of miR-181b, miR-204, and miR-599 on the gene and protein expression levels of hematopoietic cell kinase (HCK) in VSMCs.

Article Title: miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach
Article Snippet: .. After incubating the cells with PEI containing FITC-conjugated miRNA for 4 h, they were harvested and examined by flow cytometry technique (CyFlow, Partec, Germany). ..

Article Title: Potential for false-positive HIV test results using rapid HIV testing algorithms
Article Snippet: .. At the NIMR virology laboratory, the baseline assessment included: HIV confirmation using the WB technique (Immunetics Inc., Boston, Massachusetts, United States) to detect specific viral proteins for both HIV-1 and HIV-2; CD4+ cell count (cells/μL) using the flow cytometry technique employed by the Partec Cyflow Counter II (Gorlitz, Germany) to determine eligibility for the initiation of ART; and quantitation of HIV-1 RNA viral load in plasma (Roche Amplicor v1.5, Mannheim, Germany) with a lower limit of detection of 400 copies/mL. ..

Article Title: Predicted miRNAs suppressed cell proliferation and migration via FAK/VASP axis; Systems biology approach
Article Snippet: .. The FITC-labeled miRNA/PEI particles were first incubated with HT-29 cells for 4 h. Then, the cellular suspension (cold PBS) was used to evaluate the positive-FITC cells by flow cytometry technique (CyFlow, Partec, Germany). .. The cellular transfection of miRNA-PEI particles was evaluated using an electron microscope (LEO 906; Zeiss).

Article Title: In Vitro Hb Production in B-thalassemia Patients Is Not a Predictor of Clinical Responsiveness to Hydroxyurea
Article Snippet: .. Erythroid lineage maturation was assessed using monoclonal antibodies against CD71 (BD, USA) and CD235a (BD, USA) by flow cytometry technique (Partec, Cube 6, Germany) in cell culture ( ) on days 12, 14, and 16 of cell culture. ..

Article Title: Sexual Transmission of Hepatitis C Virus Between HIV Infected Subjects and Their Main Heterosexual Partners
Article Snippet: .. Measurement of CD4 counts was performed through flow cytometry technique (Partec, Germany). ..

Western Blot:

Article Title: Potential for false-positive HIV test results using rapid HIV testing algorithms
Article Snippet: .. At the NIMR virology laboratory, the baseline assessment included: HIV confirmation using the WB technique (Immunetics Inc., Boston, Massachusetts, United States) to detect specific viral proteins for both HIV-1 and HIV-2; CD4+ cell count (cells/μL) using the flow cytometry technique employed by the Partec Cyflow Counter II (Gorlitz, Germany) to determine eligibility for the initiation of ART; and quantitation of HIV-1 RNA viral load in plasma (Roche Amplicor v1.5, Mannheim, Germany) with a lower limit of detection of 400 copies/mL. ..

Cell Counting:

Article Title: Potential for false-positive HIV test results using rapid HIV testing algorithms
Article Snippet: .. At the NIMR virology laboratory, the baseline assessment included: HIV confirmation using the WB technique (Immunetics Inc., Boston, Massachusetts, United States) to detect specific viral proteins for both HIV-1 and HIV-2; CD4+ cell count (cells/μL) using the flow cytometry technique employed by the Partec Cyflow Counter II (Gorlitz, Germany) to determine eligibility for the initiation of ART; and quantitation of HIV-1 RNA viral load in plasma (Roche Amplicor v1.5, Mannheim, Germany) with a lower limit of detection of 400 copies/mL. ..

Quantitation Assay:

Article Title: Potential for false-positive HIV test results using rapid HIV testing algorithms
Article Snippet: .. At the NIMR virology laboratory, the baseline assessment included: HIV confirmation using the WB technique (Immunetics Inc., Boston, Massachusetts, United States) to detect specific viral proteins for both HIV-1 and HIV-2; CD4+ cell count (cells/μL) using the flow cytometry technique employed by the Partec Cyflow Counter II (Gorlitz, Germany) to determine eligibility for the initiation of ART; and quantitation of HIV-1 RNA viral load in plasma (Roche Amplicor v1.5, Mannheim, Germany) with a lower limit of detection of 400 copies/mL. ..

Clinical Proteomics:

Article Title: Potential for false-positive HIV test results using rapid HIV testing algorithms
Article Snippet: .. At the NIMR virology laboratory, the baseline assessment included: HIV confirmation using the WB technique (Immunetics Inc., Boston, Massachusetts, United States) to detect specific viral proteins for both HIV-1 and HIV-2; CD4+ cell count (cells/μL) using the flow cytometry technique employed by the Partec Cyflow Counter II (Gorlitz, Germany) to determine eligibility for the initiation of ART; and quantitation of HIV-1 RNA viral load in plasma (Roche Amplicor v1.5, Mannheim, Germany) with a lower limit of detection of 400 copies/mL. ..

Incubation:

Article Title: Predicted miRNAs suppressed cell proliferation and migration via FAK/VASP axis; Systems biology approach
Article Snippet: .. The FITC-labeled miRNA/PEI particles were first incubated with HT-29 cells for 4 h. Then, the cellular suspension (cold PBS) was used to evaluate the positive-FITC cells by flow cytometry technique (CyFlow, Partec, Germany). .. The cellular transfection of miRNA-PEI particles was evaluated using an electron microscope (LEO 906; Zeiss).

Suspension:

Article Title: Predicted miRNAs suppressed cell proliferation and migration via FAK/VASP axis; Systems biology approach
Article Snippet: .. The FITC-labeled miRNA/PEI particles were first incubated with HT-29 cells for 4 h. Then, the cellular suspension (cold PBS) was used to evaluate the positive-FITC cells by flow cytometry technique (CyFlow, Partec, Germany). .. The cellular transfection of miRNA-PEI particles was evaluated using an electron microscope (LEO 906; Zeiss).

Bioprocessing:

Article Title: In Vitro Hb Production in B-thalassemia Patients Is Not a Predictor of Clinical Responsiveness to Hydroxyurea
Article Snippet: .. Erythroid lineage maturation was assessed using monoclonal antibodies against CD71 (BD, USA) and CD235a (BD, USA) by flow cytometry technique (Partec, Cube 6, Germany) in cell culture ( ) on days 12, 14, and 16 of cell culture. ..

Cell Culture:

Article Title: In Vitro Hb Production in B-thalassemia Patients Is Not a Predictor of Clinical Responsiveness to Hydroxyurea
Article Snippet: .. Erythroid lineage maturation was assessed using monoclonal antibodies against CD71 (BD, USA) and CD235a (BD, USA) by flow cytometry technique (Partec, Cube 6, Germany) in cell culture ( ) on days 12, 14, and 16 of cell culture. ..



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miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow <t>cytometry.</t> Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots
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Immunophenotyping and differentiation potentials of WJ-MSCs. (A) WJ-MSCs in the Flow cytometry system, express CD44 and CD105, and are negative CD34 and CD45 markers. (B) Oil Red O staining of WJ-MSCs (C) Alizarin Red S staining of WJ-MSCs

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Exosomes of Whartons’ jelly mesenchymal stem cell reduce the NOX genes in TGF-β-induced hepatic fibrosis

doi: 10.22038/IJBMS.2022.66802.14649

Figure Lengend Snippet: Immunophenotyping and differentiation potentials of WJ-MSCs. (A) WJ-MSCs in the Flow cytometry system, express CD44 and CD105, and are negative CD34 and CD45 markers. (B) Oil Red O staining of WJ-MSCs (C) Alizarin Red S staining of WJ-MSCs

Article Snippet: The required extracted cells were examined using the well-known flow cytometry technique (Becton Dickinson, USA), and finally, the data was evaluated using FlowJo analyzer software.

Techniques: Flow Cytometry, Staining

miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow cytometry. Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots

Journal: BMC Research Notes

Article Title: miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach

doi: 10.1186/s13104-022-05920-9

Figure Lengend Snippet: miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow cytometry. Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots

Article Snippet: After incubating the cells with PEI containing FITC-conjugated miRNA for 4 h, they were harvested and examined by flow cytometry technique (CyFlow, Partec, Germany).

Techniques: Transfection, Flow Cytometry, Fluorescence